How Is Assisted Hatching Performed?
- The embryo is held with a specialized holding pipette.
- A hole is made in the ZP using either a laser or acid solution. A very delicate, hollow needle is used to expel an acidic solution against the outer “shell” (zona pellucida) of the embryo.
- A small hole is made in the shell by digesting it with the acidic solution.
- The embryo is then washed and put back in culture in the incubator.
- The embryo transfer procedure is done shortly after the hatching procedure. Embryo transfer places the embryos in the woman’s uterus where they will hopefully implant and develop to result in a live birth.
Who Should Be Treated With Assisted Hatching?
The most commonly used indications for assisted hatching with an in vitro fertilization case are:
- Women over 38 years and using their own eggs
- Women who had recurrent failure of embryo implantation
- Women whose embryos exhibit thick ZP (zona pellucida)
- Women with elevated FSH levels.
- Women with poor embryo quality